MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula1[OPEN]
نویسندگان
چکیده
Plant Biology Division, The Samuel Roberts Noble Foundation, Ardmore, OK (I.S.K., S.S., I.T.-J., C.I.P., M.K.U.); Department of Plant Biology, Carnegie Institution of Science, Stanford, CA 94305 (D.S., W.B.F.); Department of Cell and Developmental Biology, John Innes Centre, Norwich, NR4 7UH, United Kingdom (D.G., J.M.); and Division of Plant & Soil Sciences, West Virginia University, Morgantown, WV 26506 (V.A.B.)
منابع مشابه
MtSWEET11, a Nodule-Specific Sucrose Transporter of Medicago truncatula.
Optimization of nitrogen fixation by rhizobia in legumes is a key area of research for sustainable agriculture. Symbiotic nitrogen fixation (SNF) occurs in specialized organs called nodules and depends on a steady supply of carbon to both plant and bacterial cells. Here we report the functional characterization of a nodule-specific Suc transporter, MtSWEET11 from Medicago truncatula MtSWEET11 b...
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We analyzed the role of the sucrose (Suc) synthase MtSucS1 during nodulation of the model legume Medicago truncatula, integrating data for the developmental, transcriptional, and metabolic processes affected downstream of an impaired Suc cleavage in root nodules. To reduce carbohydrate supply to nodule tissues, transgenic plants expressing a p35S-driven MtSucS1-antisense fusion were constructed...
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Transgenic Medicago truncatula plants were produced harboring chimeric gene constructs of the glutamine synthetase (GS) cDNA clones (MtGS1a or MtGS1b) fused in sense or antisense orientation to the nodule-specific leghemoglobin promoter Mtlb1. A series of transgenic plants were obtained showing a 2- to 4-fold alteration in nodule GS activity when compared with control plants. Western and northe...
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Mass spectrometry (MS) has become increasingly important for tissue specific protein quantification at the isoform level, as well as for the analysis of protein post-translational regulation mechanisms and turnover rates. Thanks to the development of high accuracy mass spectrometers, peptide sequencing without prior knowledge of the amino acid sequence--de novo sequencing--can be performed. In ...
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